Premier Division side Marine welcome National League leaders Salford City, while Witton Albion’s reward for a terrific win at FC United is a home time with another National League side, Solihull Moors.
Warrington Town, who like Marine and Witton beat National League North opponents in the third qualifying round, will host Halifax Town, also of the National League.
The fourth Premier Division side still in the hat is Workington, who must get through a replay with Evo-Stik West Division side Kidsgrove Athletic on Tuesday night to earn a tie away at Hartlepool United.
The other Evo-Stik interest comes in the shape of East Division side Cleethorpes Town, who have a replay against Guiseley on Tuesday night, with the prize a home draw against Stourbridge.
The fourth qualifying round is scheduled for the weekend of Saturday October 20.
Each winning club will receive £25,000 from the FA prize fund and a place in the first round proper.
Emirates FA Cup fourth round qualifying full draw:
Cleethorpes Town or Guiseley v Stourbridge
Warrington Town v FC Halifax Town
Chorley v Barrow
Hartlepool United v Workington or Kidsgrove Athletic
AFC Fylde v Chesterfield
Southport v Ashton United
Blyth Spartans v York City
Harrogate Town v Wrexham
Dunston UTS v Gateshead
Stockport County v Altrincham
Marine v Salford City
Witton Albion v Solihull Moors
Alfreton Town v St Neots Town or Coalville Town
Woking v Welling United
Hitchin Town v Leatherhead or Hanwell Town
Chippenham Town or Hendon v Maidenhead United
Eastbourne Borough v Slough Town or Bristol Manor Farm
Hemel Hempstead Town v Oxford City
Weston Super Mare v Bath City
Boreham Wood v Dagenham & Redbridge
Tiverton Town or Metropolitan Police v Havant & Waterlooville
Gloucester City or Dorking Wanderers v Bromley
Aldershot Town v Kettering Town
Torquay United v Winchester City
Billericay Town v Taunton Town or St Albans City
Eastleigh v Hampton & Richmond Borough
Wealdstone v Sutton United
Ebbsfleet United v Worthing
Maidstone United v Leyton Orient
Haringey Borough v Horsham or Poole Town
Barnet v Braintree Town
Concord Rangers v Dover Athletic